mouse anti glur1 monoclonal antibody  (Novus Biologicals)

Journal: Cell reports

Article Title: Female-specific dysfunction of sensory neocortical circuits in a mouse model of autism mediated by mGluR5 and estrogen receptor α

doi: 10.1016/j.celrep.2024.114056

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-GluA1/GluR1 glutamate receptor , UC Davis/NIH NeuroMab Facility , Cat# N355/1; RRID:AB_2877405.

Techniques: Plasmid Preparation, Staining, Recombinant, Transfection, Immunoprecipitation, Blocking Assay, Bicinchoninic Acid Protein Assay, Expressing, Software, Imaging, Microscopy

Journal: iScience

Article Title: Necl-1/CADM3 regulates cone synapse formation in the mouse retina

doi: 10.1016/j.isci.2024.109577

Figure Lengend Snippet: Necl-1 is abundantly expressed in mouse retina Retinas from 8-week WT mice were stained with an anti-Necl-1 pAb, which recognizes the intracellular region of Necl-1 protein. (A–C) Schematic drawings of an adult mouse retina and Necl-1 localization. (A) Transverse sectional view of the retina. Light pink, retinal ganglion cells; light orange, AII BCs; light gray, amacrine cells. Orange, dark blue, and magenta arrows show signal transduction between cells. (B) Transverse sectional view of an S or S/M-opsin-containing cone pedicle and the dense plexus near the basal synapse in WT mouse retina. Expression of Necl-1 is shown in red. White circles, synaptic vesicles; light yellow, HCs; light green, ON CBCs; orange, type 4 OFF CBCs; brown, OFF CBCs; and green oval, GluA1. (C) Schematic showing Necl-1-mediated cell adhesion at the cone pedicle (left and middle) and the dense plexus near basal synapses (right). (D and E) In situ hybridization for Necl-1 . (D) Antisense probe; (E) sense probe. (F) Expression of Necl-1 in mouse retina. Lysates obtained from 8-week WT and Necl-1 −/− mouse retinas were subjected to western blotting using an anti-Necl-1 pAb and an anti-β-actin pAb. (G and H) Immunohistochemistry in Necl-1 −/− mouse retina. Retinas of 8-week WT and Necl-1 −/− mice from the same litter were subjected to immunohistochemistry using the anti-Necl-1 pAb. (I) Quantification of the Necl-1 signal detected in each stratum of the IPL. NFL, nerve fiber layer; GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; and PC, photoreceptor cell. Scale bars in D, E, G, and H, 20 μm. Error bars in I represent ±SD from WT mouse ( n = 4). See also Figure S1 .

Article Snippet: Mouse monoclonal anti-GluR1 (GluA1) , Abcam , Cat# ab31232; RRID: AB_2113447.

Techniques: Staining, Transduction, Expressing, In Situ Hybridization, Western Blot, Immunohistochemistry

Journal: iScience

Article Title: Necl-1/CADM3 regulates cone synapse formation in the mouse retina

doi: 10.1016/j.isci.2024.109577

Figure Lengend Snippet: Role of Necl-1 in localization of AMPA receptors at synapses between cones and type 4 OFF CBCs 8-week WT and Necl-1 −/− mice were used for experiments. (A) Localization of GluK1. Retinal sections were stained with an anti-GluK1 Ab and PNA. Green, GluK1; red, PNA. Scale bars, 5 μm. (B and C) Abnormal localization of GluA1 in Necl-1 −/− mouse retina. (B) Retinal sections stained with an anti-GluA1 Ab and PNA. Green, GluA1; and red, PNA. Solid arrows and dotted arrows indicate the punctate GluA1 signal. Scale bars, 5 μm. (C) The punctate GluA1 signal at the inner side of the cone pedicle. Localization of GluA1 was analyzed. Error bars represent ±SD from means of n = 7 retina sections collected from individual animals ( n = 5; WT and Necl-1 −/− , respectively). ∗∗, p < 0.001 by paired Student’s t test. (D and E) Schematic drawings of localizations of Necl-1, GluA1, and GluK1 in adult mouse retina. Transverse sectional view of the S- or S/M-opsin-containing cone pedicles in WT and Necl-1 −/− mouse retinas. Red circles, Necl-1; white circles, synaptic vesicles; light yellow, HCs; light green, ON CBCs; orange, type 4 OFF CBCs; brown, OFF CBCs; green oval, GluA1; and dark blue oval, GluK1. (D) WT; (E) Necl-1 −/− .

Article Snippet: Mouse monoclonal anti-GluR1 (GluA1) , Abcam , Cat# ab31232; RRID: AB_2113447.

Techniques: Staining

Journal: iScience

Article Title: Necl-1/CADM3 regulates cone synapse formation in the mouse retina

doi: 10.1016/j.isci.2024.109577

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-GluR1 (GluA1) , Abcam , Cat# ab31232; RRID: AB_2113447.

Techniques: Plasmid Preparation, Recombinant, Gel Extraction, PCR Cloning, Software

Journal: Brain stimulation

Article Title: Improved object recognition memory using post-encoding repetitive transcranial magnetic stimulation

doi: 10.1016/j.brs.2021.11.009

Figure Lengend Snippet: rTMS Changes Receptor Dynamics at the Synapse. Mean (+/− SEM) synaptic protein expression in the Hippocampus and Frontal Cortex of A-C) GluR1 and pGluR1(831), D-F) GluR1 and pGluR1(845), G-I) GluR2 and pGluR2(880) and J-L) TrkB and pTrkB. Overall expression and percentage phosphorylated is shown. *p<0.05 **p<0.01 ***p<0.001 vs Control, # p<0.05 vs SHAM, ## p<0.01 vs SHAM

Article Snippet: Primary antibodies used were mouse monoclonal ERK 1/2 (1:1000, Santa Cruz Biotechnology), rabbit monoclonal phospho-p44/42 MAPK (pERK 1:1000, Cell Signaling Technologies), mouse monoclonal CREB (1:500, Cell Signaling Technologies), rabbit monoclonal phospho-CREB (S133) (pCREB 1:500, Cell Signaling Technologies), rabbit monoclonal CAMKII-alpha (1:1000, Cell Signaling Technologies), mouse monoclonal phospho-CAMKII (Thr286) (pCAMKII 1:1000, Invitrogen), mouse monoclonal GluR1 (1:750, Santa Cruz Biotechnology), rabbit monoclonal phospho-GluA1 (S845) (pGluR1(845) 1:750, Cell Signaling Technologies), rabbit monoclonal phospho-GluA1 (S831) (pGluR1(831) 1:750, Cell Signaling Technologies), mouse monoclonal GluR2 (1:750, Invitrogen), rabbit polyclonal phospho-GluR2 (S880) (pGluR2 1:500, Invitrogen), mouse monoclonal TrkB (1:500, BD Transduction Laboratories) or rabbit polyclonal phospho-TrkB (Tyr816) (pTrkB 1:500, EMD Millipore).

Techniques: Expressing, Control